Ative RT-PCR analysis and 16S rRNA gene sequencing. Table S2. The list of identified proteins and their internal tryptic peptides from strain B. thuringiensis X022. Table S3. The emPAI semi-quantitative of ICPs. Table S4. The list of the proteins downregulated when Cu2+ was added. Additional file 2. Identification of B. thuringiensis proteins without and with Cu2+ added in the medium and biological replicates. Additional file 3. Functional classification of proteins identified from strain X022 without Cu2+ added in the medium. Additional file 4. Functional classification of proteins identified from strain X022 with Cu2+ added in themedium.Abbreviations qRT-PCR: quantitative RT-PCR; 2D-LC S/MS: two-dimensional liquid chromatography andem mass spectrometry; ICP: insecticidal crystal proteins; PHB: poly--hydroxybutyrate; KAS II: 3-oxoacyl-[acyl-carrier-protein] synthase 2; ALDH: aldehyde dehydrogenase; EF-G: elongation factor G; BDH:References 1. Boyce R, Lenhart A, Kroeger A, Velayudhan R, Roberts B, Horstick O. Bacillus thuringiensis israelensis (Bti) for the control of dengue vectors: systematic literature review. Trop Med Int Health. 2013;18:564?7. 2. Kaur S. Molecular approaches towards development of novel Bacillus thuringiensis biopesticides. World J Microb Biotechnol. 2000;167:81?3. 3. Sanahuja G, Banakar R, Twyman RM, Capell T, Christou P. Bacillus thuringiensis: a century of research, development and commercial applications. Plant Biotechnol J. 2011;9:283?00. 4. Tabashnik BE. Evolution of resistance to Bacillus thuringiensis. Annu Rev Entomol. 1994;39:47?9. 5. Tabashnik BE, Br ault T, Carri e Y. Insect resistance to Bt crops: lessons from the first billion acres. Nat Biotechnol. 2013;31:510?1. 6. I en Y, I en B, cengiz G. Regulation of crystal protein biosynthesis by Bacillus thuringiensis: I. Effects of mineral elements and pH. Res Microbiol. 2002;153:599?04. 7. Ozkan M, Dilek FB, Yetis U, Ozcengiz G. Nutritional and cultural parameters influencing antidipteran delta-endotoxin production. Res Microbiol. 2003;154:49?3. 8. Kurt A, Ozkan M, Ozcengiz G. Inorganic phosphate has a crucial effect on Cry3Aa delta-endotoxin production. Lett Appl Microbiol. 2005;41:303?. 9. Oves M, Khan MS, Zaidi A. Biosorption of heavy metals by Bacillus thuringiensis strain OSM29 originating from industrial effluent contaminated north Indian soil. Saudi J Biol Sci. 2013;20:121?. 10. Huang S, Ding X, Sun Y, Yang Q, Xiao X, Cao Z, et al. Proteomic analysis of Bacillus thuringiensis at different growth phases by using an automated online two-dimensional liquid chromatography andem mass spectrometry strategy. PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/16989806 Appl Environ Microbiol. 2012;78:5270?. 11. Wang J, Mei H, Zheng C, Qian H, Cui C, Fu Y, et al. The Dasatinib metabolic regulation of sporulation and parasporal crystal formation in Bacillus thuringiensis revealed by transcriptomics and proteomics. Mol Cell Proteom. 2013;12:1363?6. 12. Monro RE. Protein turnover and the formation of protein inclusions during sporulation of Bacillus thuringiensis. Biochem J. 1961;81:225. 13. Rogoff MH, Yousten AA. Bacillus thuringiensis microbiological: considerations. Annu Rev Microbiol. 1969;23:357?6.Liu et al. Microb Cell Fact (2015) 14:Page 14 of14. Chen D, Xu D, Li M, He J, Gong Y, Wu D, et al. Proteomic analysis of Bacillus thuringiensis phaC mutant BMB171/PHB-1 reveals that the PHB synthetic pathway warrants normal carbon metabolism. J Proteom. 2012;75:5176?8. 15. Raberg M, Bechmann J, Brandt U, Schl er J, Uischner B, Voig.